New biomimetic barrier Permeapad™ for efficient investigation of passive permeability of drugs

In this work the suitability of a newly invented physical patch comprising a biomimetic barrier (named Permeapad™) for drug permeability tests has been investigated. Exemplars of Permeapad™ were adapted to Franz diffusion cells and apparent permeability (Pcal_app) of a series of drugs were measured and compared with calculated partition coefficients (log Pcal_cal) of the investigated drugs as well as literature reference values obtained from Parallel Artificial Membrane Permeation Assay (PAMPA) and the cellular based method Caco-2. Furthermore, tightness of the barrier to hydrophilic marker's permeation, resistance of these barriers to proton permeation (pH changes) and shelf-life functionality were also investigated. Comparison with the published data indicated a good correlation between the permeability values measured and partition coefficients (log Pcal_cal). Moreover, a good correlation between the permeabilities measured with the new barrier and well-established in vitro Furthermore, a good correlation between the permeabilities measured with the new barrier and well-established drugs and chemical entities’ passive permeability.

- Source: di Cagno, M., Bibi, HA, & Bauer-Brandl, A. (2015). New biomimetic barrier Permeapad™ for efficient investigation of passive permeability of drugs. European Journal of Pharmaceutical Sciences, 73 , 29–34. Available at: https://doi.org/10.1016/j.ejps.2015.03.016